Targeted assays

LipidCreator — building targeted lipidomics assays

Peng B, Kopczynski D, … Hoffmann N, … Ahrends R

Nature Communications · 2020 lifs-tools.org/lipidcreator ↗

The idea

Targeted, quantitative lipidomics needs carefully designed transition lists and spectral libraries. LipidCreator provides a complete framework to define fragments, compute their masses and export ready-to-use assays — lowering the barrier to reliable quantification.

Key concepts

Computes MS/MS fragment masses for over 60 lipid classes.

Handles stable-isotope labeling and collision-energy optimization.

Generates in-silico spectral libraries and integrates with Skyline.

What we found

Assays validated both computationally and analytically.

Scales to large targeted experiments for blood-plasma analysis.

Applied to dissect lipid-signaling pathways in human platelets.