← All publications
Reference ranges
Concordant inter-laboratory ceramide concentrations in human plasma via authentic standards
The idea
Absolute lipid concentrations rarely agree between laboratories, which blocks the use of clinical reference ranges. In this inter-laboratory ring trial, participating labs quantified ceramides in human plasma reference materials using shared authentic standards — and reached concordant concentrations. Our group built the fully reproducible data integration, quantification and analysis workflow that turned the raw multi-laboratory measurements into comparable results.
Key concepts
Authentic, isotope-labelled standards give every laboratory the same calibration anchor for absolute quantification.
Human plasma reference materials provide one common sample shared across all participating sites.
A reproducible data-integration and quantification workflow harmonizes results from many instruments and labs.
What we found
Participating laboratories reported concordant absolute ceramide concentrations once authentic standards were used.
Established consensus reference concentrations for clinically relevant ceramides in human plasma reference materials.
The analysis workflow is openly released and fully reproducible at lifs-tools/ils-ceramide-ring-trial.